Characterizing Cell Adhesion by Using Micropipette Aspiration
B. Hogan, A. Babataheri, Y. Hwang, A. I. Barakat, and J. Husson
Biophysical Journal 109, 209-219 (2015) [Accès à la revue]
We have developed a technique to quantify cell-substrate adhesion force using micropipette aspiration. We position a micropipette perpendicular to the surface of an adherent cell and we apply a constant-rate aspiration pressure. We find that the detachment force scales with the cell adhesion area; this defines a critical stress, which depends on the applied aspiration rate.
Thème : Mécanique cellulaire